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The fresh nitroxide revolutionary laws enhanced in the Raw 264.7 cells sparked which have PMA than the handle tissues. That it signal is actually significantly attenuated in the tissue pretreated with mobile-impermeable SOD (Figure 1C). For every color is short for wells checked for the some other days, demonstrating the new feel of information collected for the particular weeks and you will reproducibility of your own performance across the day. The newest intensity of extracellular superoxide try dependent on subtracting the fresh rule inside the PMA cells pretreated that have SOD regarding the laws once PMA on the lack of SOD (T).
- Figure 1D depicts the fresh formula out of total and you may extracellular superoxide.
- Twist probes act effectively with ROS and produce a steady nitroxide major which is often perceived which have EPR.
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